hek blue ifn α β cells (InvivoGen)

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InvivoGen hek blue ifn α β cells
Hek Blue Ifn α β Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hek+blue+ifn+%CE%B1+%CE%B2+cells/HEK-Blue+IFN-%CE%B1+%CE%B2+cells/pm42105765-254-0-12
Average 96 stars, based on 218 article reviews

hek blue ifn α β cells - by Bioz Stars, 2026-09

96/100 stars

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Cell Culture:

Article Title: cGAS activation during human cytomegalovirus infection is driven by exogenous DNA
Article Snippet: Human dermal fibroblast (HDFn) cells obtained from Thermo Fisher Scientific were cultured in DMEM (Sigma-Aldrich, D5796) supplemented with 10 % FBS at 37 °C with 5 % CO 2 . .. HEK-Blue IFN-α/β cells obtained from InvivoGen were cultured in DMEM supplemented with 10 % FBS, 30 μg/ml blasticidin and 100 μg/ml zeocin. .. cGAS-targeting siRNA was obtained from Integrated DNA Technologies as single-stranded oligonucleotides (sense: GGAAGGAAAUGGUUUCCAAdTdT; antisense: UUGGAAACCAUUUCCUUCCdTdT)[ ] and annealed in-house.

Article Title: 5'-Triphosphate guanosine RNAs recruit GTP-binding proteins to suppress RIG-I/IFN type I signaling.
Article Snippet: Human HEK293 (ATCC, Cat#CRL-1573; RRID: CVCL_0045), HEK293 RIG-I KO, HEK293 NUDT16 KO clone 1 (C3) (Synthego), HEK293 NUDT16 KO clone 2 (C7) (Synthego), A549 (InvivoGen, Cat#a549d-nfis), A549 Dual RIG-I KO (InvivoGen, Cat#a549d-korigi), A549 Dual MDA5 KO (InvivoGen, Cat#a549d-komda5), A549 MAVS KO, and HEK-Blue IFN-α/β (InvivoGen, Cat#hkb-ifnab) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Cat#32430) supplemented with 10% fetal bovine serum (FBS; Gibco, Cat#10270-106). .. HEK-Blue IFN-α/β cells were cultured in the presence of 100 μg/mL Zeocin (InvivoGen, Cat#ant-zn-1) and 30 μg/mL Blasticidin (InvivoGen, Cat#ant-bl-05). .. Human THP-1 (ATCC, Cat#TIB-202), THP-1 Dual RIG-I KO (InvivoGen, Cat#thpdkorigi), THP-1 Dual MDA5 KO (InvivoGen, Cat#thpd-komda5), and THP-1 Dual MAVS KO (InvivoGen, Cat#thpd-komavs) cells were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, Cat#61870036) supplemented with 10% FBS.

Produced:

Article Title: CD4 T-cell platform for delivering interferons as an antiviral countermeasure with a focus on SARS-CoV-2.
Article Snippet: The viability of Vero-E6-Luc2+ cells was assessed using either CellTiter-GloTM Luminescent Cell Viability Assay Kit (Promega, Cat# PR-G7570) or OneGlo® assay (Promega, Cat #E6110). .. HEK-Blue IFN-α/β cells (InvivoGen, Cat# hkb-ifnab) or HEK-Blue IFN-λ cells (InvivoGen, Cat# hkb-ifnl) were used to quantify the amount of IFNs produced by the engineered T cells. .. Recombinant human interferon (rhIFN) proteins from R&D systems (IFN-β [Cat# 8499-IF-010/CF], IFNλ1 [Cat# 1598-IL-025/CF], IFN-λ2 [Cat# 8417-IL-025/ CF]) and IFN-α2 from InvivoGen [Cat# rcyc-hifna2b] were used as controls.

Article Title: CD4 T-cell platform for delivering interferons as an antiviral countermeasure with a focus on SARS-CoV-2
Article Snippet: The viability of Vero-E6-Luc2 + cells was assessed using either CellTiter-GloTM Luminescent Cell Viability Assay Kit (Promega, Cat# PR-G7570) or One-Glo® assay (Promega, Cat #E6110). .. HEK-Blue IFN-α/β cells (InvivoGen, Cat# hkb-ifnab) or HEK-Blue IFN-λ cells (InvivoGen, Cat# hkb-ifnl) were used to quantify the amount of IFNs produced by the engineered T cells. .. Recombinant human interferon (rhIFN) proteins from R&D systems (IFN-β [Cat# 8499-IF-010/CF], IFN-λ1 [Cat# 1598-IL-025/CF], IFN-λ2 [Cat# 8417-IL-025/CF]) and IFN-α2 from InvivoGen [Cat# rcyc-hifna2b] were used as controls.

Modification:

Article Title: Discovery of a potent anti-Zika virus benzamide series targeting the viral protein NS4B
Article Snippet: Vero 76 Cells (ATCC CRL-158) were maintained in Modified Eagle’s Medium with Earle’s Balanced Salt Solution and L-glutamine (MEM-E) supplemented with 10% fetal bovine serum (FBS) (Corning CellGro). .. Huh-7.5.1 cells (a gift from Dr. Andrew Tai, University of Michigan), HEK 293T cells (BEI, NR-9313), and HEK-Blue IFN-α/β cells (InvivoGen) were maintained in Dulbecco’s Modified Eagle Medium with 4.5g/L glucose, L-glutamine, and sodium pyruvate supplemented with 10% FBS. ..

Article Title: Discovery of a potent anti-Zika virus benzamide series targeting the viral protein NS4B.
Article Snippet: Vero 76 Cells (ATCC CRL-158) were maintained in Modified Eagle’s Medium with Earle’s Balanced Salt Solution and L-glutamine (MEM-E) supplemented with 10% fetal bovine serum (FBS) (Corning CellGro). .. Huh-7.5.1 cells (a gift from Dr. Andrew Tai, University of Michigan), HEK 293T cells (BEI, NR-9313), and HEK-Blue IFN-α/β cells (InvivoGen) were maintained in Dulbecco’s Modified Eagle Medium with 4.5g/L glucose, L-glutamine, and sodium pyruvate supplemented with 10% FBS. ..

Incubation:

Article Title: Discovery of a potent anti-Zika virus benzamide series targeting the viral protein NS4B
Article Snippet: .. The following day, 20 μL of the cell supernatant from the HEK-Blue IFN-α/β cells were further incubated with 180 μL of pre-warmed Quanti-blue SEAP reagent (Invivogen) for 2 hours and the O.D. .. A620 was measured using a Cytation 1 (Agilent).

Article Title: Discovery of a potent anti-Zika virus benzamide series targeting the viral protein NS4B.
Article Snippet: .. The following day, 20 μL of the cell supernatant from the HEK-Blue IFN-α/β cells were further incubated with 180 μL of pre-warmed Quanti-blue SEAP reagent (Invivogen) for 2 hours and the O.D. .. A620 was measured using a Cytation 1 (Agilent).

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